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Dark Quencher Phosphoramidites

Dark Quencher – 534 Phosphoramidites


S. No Catalog# Product name Combination CAS No MSDS Download
1 See Description Dark Quencher – 534 Phosphoramidites Dyes View

Dark Quencher-579 Phophoramidites


S. No Catalog# Product name Combination CAS No MSDS Download
1 See Description Dark Quencher-579 Phophoramidite Dyes View

Dark Quencher-DMT-dU-C-5-579 Phosphoramidite


S. No Catalog# Product name Combination CAS No MSDS Download

Dark Quencher-534 Phosphoramidites & Dark Quencher-579 Phosphoramidites are a non-fluorescent chromophore, and extensively used as the 3'-quencher moiety in a variety of FRET (Fluorescence Resonance Energy Transfer) in DNA detection probes. Such probes are primarily used in nucleic acid assays.

Advantages:

The advantages of using a Dark Quencher in a FRET probe are

  • Low background fluorescence & higher dynamic range,
  • Responsiveness to multiplex assays - because of a dark quencher having no secondary fluorescence.
  • Comfort of synthesis of FRET probes with a Dark Quencher - because dark quenchers being resistant to degradation throughout the oligo deprotection step.

Storage and handling:
Shipping conditions: Cold Box Storage conditions: -15 to -30 °C, Light sensitive material.

Product Name Another Name
Dark Quencher 534 Phosphoramidite BHQ 534 Phosphoramidite
Dark Quencher 579 Phosphoramidite BHQ 579 Phosphoramidite
Dark Quencher-DMT-dU-C-5-579 Phosphoramidite BHQ DMT-dU-C-5- 579 Phosphoramidite